ADAM12 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60740-50, BS60740-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43184

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: ADAM (a disintegr in and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNF (tumor necrosis factor α), and modulation of the neurogenic function of Notch and Delta. ADAM proteins possess a signaldomain, a pro-domain, a metalloprotease domain, a disintegrin domain (integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone and muscle, and catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAM12 (Meltrin-α) is produced as 2 differentially spliced isoforms, a 718 amino acid secreted form (ADAM12S) and a 881 amino acid membrane-bound form (ADAM12L), and is involved in egg-sperm fusion.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ADAM12 polyclonal antibody detects endogenous levels of ADAM12 protein.

Bioworld Molecular Weight: ~ 100 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ADAM12 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of ADAM12 pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic, membrane and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ADAM12 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50, showing cytoplasmic, membrane and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Disintegrin and metalloproteinase domain-containing protein 12; ADAM 12; Meltrin-alpha; ADAM12; MLTN; UNQ346; PRO545

Immunogen: A synthetic peptide corresponding to residues in Human ADAM12

Conjugate: Unconjugated

Modification: Unmodified