ADCY9 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS5590-50, BS5590-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60503

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000
IHC:1:50~1:200

Background: Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules (primarily cyclic AMP-dependent protein kinases) to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Classically, adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor (i.e., α β-adrenergic receptor) catalyzes the exchange of GDP (bound to Gα s) for GTP, dissociation of GTP-Gα s
from Gβ γ and Gα s-mediated activation of adenylyl cyclase. The most abundant cerebral adenylyl cyclases appears to be adenylyl cyclase IX. AC IX is confined to the gray matter and its expression is mainly neuronal, with its highest expression located at the hippocampus. ACIX is also expressed in heart, pancreas and thyrocytes. AC I and AC IX are regulated reciprocally by intracellular free Ca2+. The inhibition of AC IX by Ca2+ is blocked by the calcineurin inhibitors FK506 and cyclosporin A.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ADCY9 polyclonal antibody detects endogenous levels of ADCY9 protein.

Bioworld Molecular Weight: ~ 161 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ADCY9 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of ADCY9 pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ADCY9 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Adenylate cyclase type 9; ATP pyrophosphate-lyase 9; Adenylate cyclase type IX; Adenylyl cyclase 9; ADCY9; KIAA0520

Immunogen: A synthetic peptide corresponding to residues in Human ADCY9.

Conjugate: Unconjugated

Modification: Unmodified