HA-tag (4G3) Monoclonal Antibody-HRP

Sizes: 50µl, 100µl

Catalogue Numbers: AP0005MH-50, AP0005MH-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Mouse

Reactivity: All

Applications: WB, IF, IP,

All Applications: WB:1:1000~1:5000
IF:1:1000
IP:1:100

Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HA-tag (4G3) monoclonal antibody-HRP detects over-expressed proteins containing the HA epitope tag.

Bioworld Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HA-tag (4G3) mAb-HRP at 1:10000 dillution
Lane 1: HEK293T whole cell lysate ,untransfected (40μg)
Lane 2: HEK293T whole cell lysate, transfected with pCMV-HA-IMPDH2(WT) (40μg)

Alternative Name: HA epitope tag; HA Tag; hemagglutinin

Immunogen: Synthetic peptide HA-tag.

Conjugate: HRP

Modification: Unmodified