Tubulin α (9H4) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB8025-50, MB8025-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q71U36/ P68363

Host: Mouse

Reactivity: Human, Mouse, Rat, Odontobutis obscurus

Applications: WB, IF

All Applications: WB: 1:5000~1:20000
IF: 1:500~1000

Background: Tubulin is a major cytoskeleton component that has three distinct forms, designated α, β and γ Tubulin. α and β Tubulins form heterodimers, which multimerize to form a microtubule filament. γ Tubulin forms a soluble multiprotein particle with several other proteins. This particle, designated the gammasome, is required for nucleating microtubule filaments at the centrosome. In several organisms, numerous isoforms of the Tubulins exist that are encoded by different genes. The α and β isoforms undergo a variety of post-translational modifications, which may affect microtubule stability and protein interactions. High expression of class II β Tubulin has been seen in elongating axons, indicating a role in neurite outgrowth. Tubulins may also play a role in non-neuronal cell process formation

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: α-tubulin (9H4) mAb detects endogenous levels of α-tubulin protein.

Bioworld Molecular Weight: ~ 55 KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of α-tubulin (9H4) mAb at 1:5000/10000/20000 dilution
Lane 1: The Kidney tissue lysate of Mouse (20ug)
Lane 2: The Kidney tissue lysate of Rat (20ug)
Lane 3: PC12 whole cell lysate (20ug)
Lane 4: CT-26 whole cell lysate (20ug)
Lane 5: HEK293T whole cell lysate (20ug), Immunofluorescence analysis of RAW264.7 cells using α-tubulin (9H4) mAb at dilution of 1:1000 (40x lens).

Alternative Name: Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1A; TUBA3; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B

Immunogen: Synthetic peptide, corresponding Human α-tubulin.

Conjugate: Unconjugated

Modification: Unmodified