CstF-64 (R46) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1980-50, BS1980-100

Sizes: 50µl, 100µl

Swiss-Prot: P33240

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CstF-64 (R46) polyclonal antibody detects endogenous levels of CstF-64 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CstF-64 (R46) pAb at 1:1000 dilution
Lane 1: K562 whole cell lysate (20ug)
Lane 2: HEK293T whole cell lysate (20ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: SP2/0 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of CstF-64 (R46) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 64 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cleavage stimulation factor 64 kDa subunit; CSTF 64 kDa subunit; CF-1 64 kDa subunit; CstF64; CstF 64; CSTF2;

Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human CstF-64.

Conjugate: Unconjugated

Modification: Unmodified