MMP-1 (H440) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1229-50, BS1229-100

Sizes: 50µl, 100µl

Swiss-Prot: P03956

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF

Background: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is downregulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MMP-1 (H440) polyclonal antibody detects endogenous levels of MMP-1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MMP-1 (H440) pAb at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: CT26 whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug)
Lane 5: HepG2 whole cell lysate (40ug), Western blot (WB) analysis of MMP-1 (H440) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (30ug)
Lane 2: The kidney tissue lysate of Mouse (40ug)
Lane 3: The kidney tissue lysate of Rat (15ug)
Lane 4: L02 whole cell lysate (30ug)

Bioworld Molecular Weight: ~ 54 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1; 22 kDa interstitial collagenase; 27 kDa interstitial collagenase; MMP1; CLG

Immunogen: Synthetic peptide, corresponding to amino acids 411-460 of Human MMP-1.

Conjugate: Unconjugated

Modification: Unmodified