KSP Cadherin Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB66077-50, MB66077-100 Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Swiss-Prot: O75309 Host: Mouse Reactivity: Human Applications: IHC All Applications: IHC (1/100 - 1/300) Background: The cadherins are a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. Cadherin-16, also known as Ksp-cadherin, is a type I membrane protein which is kidney-specific. Cadherin-16 is expressed exclusively in the basolateral membrane of renal tubular epithelial cells. The human Ksp-cadherin gene (cadherin-16) maps to chromosome 16q22.1. The mouse Ksp-cadherin gene was localized to a highly syntenic region of distal chromosome 8. Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Recognizes endogenous levels of KSP Cadherin protein. Bioworld Molecular Weight: ~ 89 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Immunohistochemical analysis of KSP Cadherin staining in human chromophobe cell renal carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of KSP Cadherin staining in human renal clear cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Alternative Name: Cadherin-16; Kidney-specific cadherin; Ksp-cadherin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human KSP Cadherin. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified