CD34 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0202P-50, NCP0202P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P28906

Host: Rabbit

Reactivity: Human

Applications: WB, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200

Background: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD34 polyclonal antibody detects endogenous levels of CD34 protein.

Bioworld Molecular Weight: ~ 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD34 polyclonal antibody at 1:1000 dilution
Lane 1: A549 cell membrane lysate (30ug)
Lane 2: THP-1 whole cell lysate (30ug), Immunofluorescence analysis of A549 cells using CD34 pAb at dilution of 1:200.

Alternative Name: Hematopoietic progenitor cell antigen CD34; CD34

Immunogen: Recombinant protein of human CD34.

Conjugate: Unconjugated

Modification: Unmodified