RGS20 Polyclonal Antibody

Catalogue Numbers: BS60230-50, BS60230-100

Sizes: 50µl, 100µl

Alternative Name: Regulator of G-protein signaling 20; Gz-selective GTPase-activating protein; G(z)GAP; Gz-GAP; Regulator of G-protein signaling Z1; Regulator of Gz-selective protein signaling 1; RGSZ1; ZGAP1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O76081

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The regulators of G protein signaling (RGS) proteins inhibit heterotrimeric G protein signaling. RGS proteins work by functioning as GTPase-activating proteins (which increase the GTPase activity of G protein a subunits) thereby driving G proteins into their inactive GDP-bound form. RGS20 is expressed exclusively in brain, with highest levels in the caudate nucleus and temporal lobe. RGS20 belongs to the RZ subfamily because it is highly selectivie for the az subunit on G proteins. However, if protein kinase C phosphorylates the az subunit, the G protein is much less susceptible to RGS20 action. RGS20 directly interacts with the microtubule-destabilizing protein SCG10 (superior cervical ganglia, neural specific 10) and blocks its ability to induce microtubule disassmebly.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RGS20 polyclonal antibody detects endogenous levels of RGS20 protein.

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RGS20 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human RGS20.

Conjugate: Unconjugated

Modification: Unmodified