RPAP1 Polyclonal Antibody

Catalogue Numbers: BS60238-50, BS60238-100

Sizes: 50µl, 100µl

Alternative Name: RNA polymerase II-associated protein 1; RPAP-1; KIAA1403

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BWH6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: RNA polymerases transcribe nuclear genes for ribosomal RNA and are integral components of ribosomal biogenesis. RNA polymerase I (Pol I) is located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. RNA polymerase II (Pol II) synthesizes mRNA. RNA polymerase III (Pol III) transcribes class III genes, encoding a number of small ribosomal RNA molecules including tRNA and 5S rRNA. RPAP1 (RNA polymerase II associated protein 1) is a 1,393 amino acid nuclear protein that is thought to connect Pol II to acetylated Histone H3 and regulators of protein complex formation. A member of the RPAP1 family, RPAP1 exists as three alternatively spliced isoforms that are encoded by a gene that maps to human chromosome 15q15.1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RPAP1 polyclonal antibody detects endogenous levels of RPAP1 protein.

Bioworld Molecular Weight: ~ 153 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RPAP1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human RPAP1.

Conjugate: Unconjugated

Modification: Unmodified