CES2 Polyclonal Antibody

Catalogue Numbers: BS5659-50, BS5659-100

Sizes: 50µl, 100µl

Alternative Name: Cocaine esterase; Carboxylesterase 2; CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2; CES-2; ICE

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00748

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: CES1 and CES2 are the two major liver carboxylesterases which belong to the type-B carboxylesterase/lipase family. Helping the body in the detoxification of a wide range of xenobiotics, CES1 and CES2 are involved in the hydrolyzing activation of therapeutic ester and amide pro-drugs, as well as in the detoxification of several narcotic compounds. The catalytic activity of CES1 and CES2 is influenced by both the esterification site and the structure/moiety of the amino acid. While CES1 shows high affinity for aromatic and aliphatic esters, CES2 shows high affinity for 3,6-diacetyl and 6-monoacetly esters, such as those found in morphine and morphine derivatives.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CES2 polyclonal antibody detects endogenous levels of CES2 protein.

Bioworld Molecular Weight: ~ 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CES2 (Q369) pAb at 1:500 dilution
Lane 1: HepG2 whole cell lysate(40ug)
Lane 2: HCT116 whole cell lysate(40ug)
Lane 3: AML-12 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 379-424 of Human CES2.

Conjugate: Unconjugated

Modification: Unmodified