CYP11A1 Polyclonal Antibody

Catalogue Numbers: BS5680-50, BS5680-100

Sizes: 50µl, 100µl

Alternative Name: Cholesterol side-chain cleavage enzyme, mitochondrial; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc); CYP11A1; CYP11A

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05108

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: CYP11A1, also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA, is an enzyme that catalyzes the first step of steroid biosynthesis under the modulation of cAMP signal. CYP11A1 in steroidogenic cells converts cholesterol to pregnenolone, which is determined by hormonal control of cholesterol availability. Expression of the CYP11A1 gene is controlled by the transcription factor SF-1, and the upstream SF-1 binding site in the CYP11A1 gene is required for hormonal stimulation. c-Jun and SF-1 may act synergistically to activate CYP11A1 gene expression. Both Forskolin and 8-Br-cAMPS elevate CYP11A1 mRNA levels in the interstitial cell monolayer, which has a fully functional adenylate cyclase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP11A1 polyclonal antibody detects endogenous levels of CYP11A1 protein.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP11A1 polyclonal antibody at 1:500 dilution
Lane 1: Jurkat cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat liver tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 457-502 of Human CYP11A1.

Conjugate: Unconjugated

Modification: Unmodified