USP19 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5975-50, BS5975-100

Sizes: 50µl, 100µl

Swiss-Prot: O94966

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. Ubiquitin-specific-processing protease 19 (USP19), also known as ubiquitin carboxyl-terminal hydrolase 19, ubiquitin thioesterase 19 and deubiquitinating enzyme 19, is a 1318 amino acid member of the peptidase C19 family of proteins. USP19 is thought to be involved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome. Four known isoforms of USP19 exist as a result of alternative splicing events.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: USP19 polyclonal antibody detects endogenous levels of USP19 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of USP19 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: HEK293T cell lysate
Lane 3: H9C2 cell lysate, Immunohistochemistry (IHC) analyzes of USP19 pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing endoplasmic reticulum membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 146 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Ubiquitin carboxyl-terminal hydrolase 19; Deubiquitinating enzyme 19; Ubiquitin thioesterase 19; Ubiquitin-specific-processing protease 19; Zinc finger MYND domain-containing protein 9; USP19; KIAA0891; ZMYND9

Immunogen: Synthetic peptide, corresponding to amino acids 439-484 of Human USP19.

Conjugate: Unconjugated

Modification: Unmodified