eIF3ε (V116) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3296-50, BS3296-100

Sizes: 50µl, 100µl

Swiss-Prot: O00303

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFε, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: eIF3ε (V116) polyclonal antibody detects endogenous levels of eIF3ε protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of eIF3ε (V116) pAb at 1:1000 dilution
Lane 1: The Testis tissue lysate of Mouse (40ug)
Lane 2: The Testis tissue lysate of Rat (40ug)
Lane 3: Hela whole cell lysate (40ug)
Lane 4: Panc1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of eIF3ε (V116) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: EIF3S5; EIF3F; Eukaryotic translation initiation factor 3 subunit F; eIF3f; Deubiquitinating enzyme eIF3f; EC=3.4.19.12; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47

Immunogen: Synthetic peptide, corresponding to amino acids 91-140 of Human eIF3ε.

Conjugate: Unconjugated

Modification: Unmodified