Cdc25B Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90267-50, BS90267-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P30305(Human)

Host: Rabbit

Reactivity: Human

Applications: WB IP

All Applications: WB:1:1,000-1:2,000

Background: The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while Tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 Tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a Tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other Tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cdc25B on THP-1 cellsLysates using anti-Cdc25B antibody at 1/1,000 dilution.

Bioworld Molecular Weight: 65 kDa

Specificity: Cdc25B polyclonal antibody detects endogenous levels of Cdc25B protein.

Alternative Name: CDC25B, AI604853, Cdc 25B, Cdc25b, CDC25HU2, Cdc25m2, Cell division cycle 25 homolog B, Cell division cycle 25B, Cell division cycle 25B isoform 1, Cell division cycle 25B isoform 2, Cell division cycle 25B isoform 3, Cell division cycle 25B isoform 4, Cell division cycle 25B isoform 5, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M phase inducer phosphatase 2, M-phase inducer phosphatase 2, MPIP2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified