NMDAR1(Phospho-Ser890)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65216-50, BS65216-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q05586

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC-P, IF/ICC, ELISA

All Applications: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Background: glutamate ionotropic receptor NMDA type subunit 1(GRIN1) Homo sapiens The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-NMDAζ1 (S890) Polyclonal Antibody detects endogenous levels of NMDAζ1 protein only when phosphorylated at S890.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide., Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NMDAR1 (Phospho-Ser890) Antibody, Immunofluorescence analysis of A549 cells, using NMDAR1 (Phospho-Ser890) Antibody. The picture on the right is blocked with the phospho peptide., Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR1 (Phospho-Ser890) Antibody. The picture on the right is blocked with the phospho peptide.

Alternative Name: GRIN1; NMDAR1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1

Immunogen: The antiserum was produced against synthesized peptide derived from human NMDAR1 around the phosphorylation site of Ser890. AA range:856-905

Conjugate: Unconjugated

Modification: Phosphorylation