Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Sizes: 48T, 96T, 500 Assays

Catalogue Numbers: E-BC-K125-M-48, E-BC-K125-M-96, E-BC-K125-M-500

Citations, Manuals and MSDS Available upon request.

Target Synonym: ChAT

Research Areas: Amino Acids and Proteins

Result Type: Enzyme Activity

Detection Instrument: Microplate reader

Tested Sample Types: Animal tissue

Assay Time: 65 min

Detection Type: Colorimetric method

Sensitivity: 1.21 U/g fresh weigh

Detection Range: 1.21-40 U/g fresh we

Test Principle: Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA) , CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.

Inter CV: 9.4

Intra CV: 5.1

Storage: This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Shipping: Ice bag