Histone H3.3 (K27) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1752-50, BS1752-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P84243

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of Histone H3. 3 (K27) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 16 kDa

Specificity: Histone H3.3 (K27) polyclonal antibody detects endogenous levels of Histone H3.3 protein.

Alternative Name: Histone H3.3; H3F3A; H3.3A; H3.3B; H3F3; H3F3B

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Histone H3.3.

Conjugate: Unconjugated

Modification: Unmodified