Dbf4 (I18) Polyclonal Antibody

Catalogue Numbers: BS2372-50, BS2372-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UBU7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Dbf4 (I18) polyclonal antibody detects endogenous levels of Dbf4 (I18) protein.

Extra Notes: Western blot (WB) analysis of Dbf4 (I18) pAb at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)
Lane3:The Testis tissue lysate of Mouse(40ug)
Lane4:The Testis tissue lysate of Rat(40ug)

Bioworld Molecular Weight: ~ 77 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1; DBF4; ASK; DBF4A; ZDBF1

Immunogen: Synthetic peptide, corresponding to the N-terminual of Human Dbf4.

Conjugate: Unconjugated

Modification: Unmodified