nNOS Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90953-50, BS90953-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29475(Human) Q9Z0J4(Mouse) P29476(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: nNOS polyclonal antibody detects endogenous levels of nNOS protein.

Bioworld Molecular Weight: 161 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of nNOS on mouse heart lysates using anti-nNOS antibody at 1/1,000 dilution., ICC staining nNOS in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: N-NOS, nNOS, NOS1, 2310005C01Rik, BNOS, Constitutive NOS, EC 1.14.13.39, IHPS 1, IHPS1, N-NOS, NC-NOS, neuronal Nitric Oxide Synthase, Neuronal NOS, Nitric oxide synthase , neuronal, included, Nitric oxide synthase 1 (neuronal), Nitric oxide synthase 1, Nitric oxide synthase, brain, Nitric oxide synthase, penile neuronal, included, NNOS, NO, NOS 1, NOS, NOS type I, NOS-I, NOS1, NOS1, Peptidyl-cysteine S-nitrosylase NOS1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified