Phospho-JAK2-Y1007 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79457-50, BS79457-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60674

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:200 - 1:2000

Background: This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-JAK2-Y1007 antibody at 1:760 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Bioworld Molecular Weight: 125KDa

Specificity: Phosphorylated

Alternative Name: JAK2; JTK10; THCYT3

Immunogen: A synthetic phosphorylated peptide around Y1007 of human JAK2(NP_004963.1).

Conjugate: Unconjugated

Modification: Phosphorylated