AKR1C1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9044-50, MB9044-100

Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q04828(Human)

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, IHC ICC FC

All Applications: WB:1:2,000-1:10,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Human liver contains isoforms of dihydrodiol dehydrogenase (DD1, DD2, DD3 and DD4), which belong to the aldo-oxo reductase / aldo-keto reductase (AKR) superfamily, have 20α- or 3α-hydroxysteroid dehydrogenase AKD1C3 and 3α-HSD are alternate designations for human DD3 (which is referred to as AKR1C18 in rodents), while DD4 is also designated as AKR1C1, DDH or DDH1, while DD2 also can be designated AKR1C2, dDD, BABP or DDH2. AKR1C4, CD, CHDR or AKR1C6 (in rodents). DD1 and DD2 are 20α-HSDs, DD3 and DD4 are the 3α-HSDs. The 20α-HSD catalyzes the reaction of progesterone to the inactive form 20α-hydroxyprogesterone. The 3α-HSD is a cytosolic, monomeric, NADPH-depen DD1 and DD2 are ubiquitously expressed, whereas DD4 mRNA is restricted to the liver. DD3 is a unique enzyme that can specifically catalyze the dehydrogenation of trans-benzene dihydrodiol and trans- naphthalenedihydrodiol.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: AKR1C1 monoclonal antibody detects endogenous levels of AKR1C1 protein.

Bioworld Molecular Weight: 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Human liver tissue, ICC staining AKR1C1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: 20-alpha-HSD, DD1/DD2, HBAB, AKR1C1, DDH, DDH1, 2-dihydrobenzene-1, 2-diol dehydrogenase, 20 alpha (3 alpha) hydroxysteroid dehydrogenase, 20 ALPHA HSD, 20 alpha hydroxysteroid dehydrogenase, 20-alpha-HSD, 20-alpha-hydroxysteroid dehydrogenase, 20ALPHAHSD, 2ALPHAHSD, AK1C1, AK1C1, AKR1C1, Aldo keto reductase family 1 member C1, aldo-keto reductase C, Aldo-keto reductase family 1 member C1, Aldo-keto reductase family, 1 member 1, C9, Chlordecone reductase homolog, Chlordecone reductase homolog HAKRC, DD1, DD1/DD2, DDH, DDH1, Dihydrodiol dehydrogenase 1, Dihydrodiol dehydrogenase 1/2, dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase isoform DD1, Dihydrodiol dehydrogenase, type 1, H37, HAKRC, HBAB, Hepatic dihydrodiol dehydrogenase, High affinity hepatic bile acid-binding protein, High-affinity hepatic bile acid-binding protein, Indanol dehydrogenase, MBAB, MGC8954, Trans-1, Trans-1, 2 dihydrobenzene 1, 2 diol dehydrogenase, Type II 3 alpha hydroxysteroid dehydrogenase,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified