SETD3 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS78246-50, BS78246-100

Swiss-Prot: Q86TU7

Host: Rabbit

SETD3 Polyclonal Antibody-BS78246

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,50 - 1:200

Background: Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SETD3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug SETD3 antibody. Western blot was performed from the immunoprecipitate using SETD3 antibody at a dilution of 1:1000.

Alternative Name: SETD3; C14orf154; SET domain containing 3

Immunogen: A synthetic peptide of human SETD3(NP_115609.2).

Modification: Unmodified

Conjugate: Unconjugated