HYD (T14) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1460-50, BS1460-100

Sizes: 50µl, 100µl

Swiss-Prot: O95071

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: HYD (T14) polyclonal antibody detects endogenous levels of HYD protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HYD (T14) polyclonal antibody at 1:500 dilution
Lane 1: CT26 whole cell lysate (40ug)
Lane 2: PC12 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: HK-2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of HYD (T14) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 309 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; Hyperplastic discs protein homolog; hHYD; Progestin-induced protein; UBR5; EDD; EDD1; HYD; KIAA0896

Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human HYD.

Conjugate: Unconjugated

Modification: Unmodified