M-RIP Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA27377-50, RA27377-100

Citations, Manuals and MSDS Available upon request.

Background: function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.,similarity:Contains 1 PH domain.,similarity:Contains 2 PH domains.,subcellular location:Colocalizes with F-actin.,subunit:Binds F-actin through its N-terminus (By similarity) . Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.,

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Alternative Names: MPRIP; KIAA0864; MRIP; RHOIP3; Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip

Applications: IHC-p; IF (paraffin section) ; WB; ELISA

Species Cross-Reactivity: Human; Mouse; Rat

GeneID (Human): 23164

Protein MW (KDa): 110

SWISS: Q6WCQ1

Source: Rabbit

Research Use Only