Human CA15-3 (Carbonhydrate Antigen 15-3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0245-48, ELK0245-96, ELK0245-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CA15-3

Assay Type: Sandwich

Sensitivity: 0.47 U/mL

Standard: 100 U/mL

Detection Range: 1.57-100 U/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Tumor immunity; Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only