Recombinant Human Carbonic Anhydrase 8/CA8 Protein (His Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032165-10, PKSH032165-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Carbonic Anhydrase 8; CA8

Target Symptom: Carbonic Anhydrase-Related Protein; CARP; Carbonic Anhydrase VIII; CA-VIII; CA8; CALS; CAMRQ3; MGC120502; MGC99509

Research Area: Cancer

Target Species: Human

Expression Host: E.coli

Application: Enzyme

Fusion Tag: C-6His

UNIProt ID: P35219

Accession: P35219

Background: Carbonic Anhydrase 8 (CA8) belongs to the alpha-carbonic anhydrase family. Alpha-carbonic anhydrase is a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. Because CA8 has some sequence similarity with other known carbonic anhydrase genes, it was firstly called as CA-related protein. Nevertheless, CA8 does not have a carbonic anhydrase catalytic activity. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3), which is a congenital cerebellar ataxia associated with dysarthia, quadrupedal gait and mild mental retardation.

Activity: Measured by its esterase activity The specific activity is 162. 5 pmol/min/ug.

Sequence: Ala2-Gln290

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 1mM DTT, pH 8.5 .
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 34.0 kDa

Observed MW: 40 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only