PAK4/5/6 (V469) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3247-50, BS3247-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O96013/Q9NQU5/Q9P286

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB: 1:500~1:1000
IP: 1:10~1:100

Background: PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PAK4/5/6 (V469) polyclonal antibody detects endogenous levels of PAK4, PAK5 and PAK6 protein.

Bioworld Molecular Weight: ~ 64 kDa (PAK4), 80 kDa (PAK5), 75 kDa (PAK6)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PAK4/5/6 (V469) pAb at 1:500 dilution
Lane 1: H1792 whole cell lysate (20ug)
Lane 2: MCF-7 whole cell lysate (20ug)
Lane 3: PC3 whole cell lysate (20ug)
Lane 4: The Testis tissue lysate of Mouse (40ug)
Lane 5: The Testis tissue lysate of Rat (40ug), Immunoprecipitation of HEK293T cell lysate using PAK4/5/6 (V469) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using PAK4/5/6 (V469) #BS3247. “↑”（supernatant）;“↓(deposition）

Alternative Name: Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; PAK4; KIAA1142; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6; PAK-6; PAK6; PAK5; Serine/threonine-protein kinase PAK 7; p21-activated kinase 5; p21-activated kinase 7; PAK-7; PAK7; KIAA1264

Immunogen: Synthetic peptide, corresponding to amino acids 441-490 of Human PAK4.

Conjugate: Unconjugated

Modification: Unmodified