Aspartate Aminotransferase (AST/GOT) Activity Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F043-48, E-BC-F043-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: AST;GOT

Research Areas: Liver and Renal Function

Result Type: Enzyme Activity

Detection Instrument: Fluorescence microplate reader

Tested Sample Types: cell, animal tissue, serum (plasma) and other liquid samples

Assay Time: 80 min

Detection Type: Fluorescence method

Sensitivity: 0.03 U/L

Detection Range: 0.03-1.5 U/L

Test Principle: AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.

Inter CV: 7.3

Intra CV: 2.2

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag