MSH2 Monoclonal Antibody

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB66986-50, MB66986-100

Sizes: 50µl, 100µl

Swiss-Prot: P43246

Host: Mouse

Reactivity: Human

Applications: WB, IHC

Background: The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between non-identical DNA sequences and induces both checkpoint and apoptotic responses following certain types of DNA damage . MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex .
Mutations in MSH2 have been found in a large proportion of hereditary non-polyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world . Mutations have also been associated with other sporadic tumors.

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of MSH2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MSH2 expression in 293 (A), Hela (B) whole cell lysates., Immunohistochemical analysis of MSH2 staining in human appendix formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 105 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2

Immunogen: Recombinant fusion protein of human MSH2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified