CD144 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0269P-50, NCP0269P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P33151

Host: Rabbit

Reactivity: Human

Applications: WB, IP,

All Applications: WB: 1:1000~1:2000
IP 1:50 - 1:200

Background: Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking. Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers. Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD144 polyclonal antibody detects endogenous levels of CD144 protein.

Bioworld Molecular Weight: ~120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD144 polyclonal antibody at 1:1000 dilution
Lane 1: SGC7901 whole cell lysate (3ug)
Lane 2: HEPG2 whole cell lysate (30ug)
Lane 3: HEK293T whole cell lysate (30ug), Immunoprecipitation of HepG2 cell lysates using CD144 pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using CD144 pAb #NCP0269P.

Alternative Name: Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CD144; CDH5

Immunogen: Synthetic peptide, corresponding to Human CD144.

Conjugate: Unconjugated

Modification: Unmodified