CXXC1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS75331-50, BS75331-100

Swiss-Prot: Q9P0U4

Host: Rabbit

CXXC1 Polyclonal Antibody-BS75331

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:20 - 1:50

Background: This gene encodes a protein that functions as a transcriptional activator that binds specifically to non-methylated CpG motifs through its CXXC domain. The protein is a component of the SETD1 complex, regulates gene expression and is essential for vertebrate development.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 80kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CXXC1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s., Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 3ug CXXC1 antibody. Western blot was performed from the immunoprecipitate using CXXC1 antibody at a dilution of 1:500.

Alternative Name: CXXC1; 2410002I16Rik; 5830420C16Rik; CFP1; CGBP; HsT2645; PCCX1; PHF18; SPP1; ZCGPC1; hCGBP

Immunogen: Recombinant fusion protein of human CXXC1(NP_001095124.1).

Modification: Unmodified

Conjugate: Unconjugated