Recombinant Human ACO1/irp1 Protein (His Tag)

Size: 50μg

Catalogue Numbers: PKSH031334-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: ACO1; irp1

Target Synonym: ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1

UNIProt ID: P21399

Research Areas: Signal Transduction; Cardiovascular; Cancer; epigenetics and nuclear signal; metabolism;

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Fusion Tag: N-His

Accession: P21399

Background: Aconitase 1(ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur(Fe-S) isomerases and two types of iron regulatory protein. Aconitase exits in two forms: one is soluble and the other is mitochondrial. ACO1 is the soluble existing form, and the mitochondrial form is ACO2. Residues from all three N-terminal domains and the larger C-terminal domain contribute to the active site region. When the enzyme is activated, it gains an additional iron atom. ACO1 can assume two different functions in cells, depending on different conditions. During iron scarcity or oxidative stress, ACO1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, ACO1 binds an iron-sulfur cluster to function as a cytosolic aconitase.

Sequence: Met 1-Lys 889

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 2mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 101 kDa

Observed MW: 90 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only