Recombinant Mouse ENPEP/Aminopeptidase A Protein (His Tag)(Active)

Size: 50μg

Catalogue Number: PKSM040935-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: ENPEP; Aminopeptidase A

Target Symptom: 6030431M22Rik; APA; Bp-1/6C3; ENPEP; Ly-51; Ly51

Target Species: Mouse

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion Tag: N-His

UNIProt ID: P16406

Accession: P16406

Background: ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.

Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin. The specific activity is > 200 pmoles/min/μg.

Sequence: Arg41-Pro945

Purity: > 91 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 106 kDa

Observed MW: 113 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only