PEBP1 Polyclonal Antibody

Catalogue Numbers: BS5912-50, BS5912-100

Sizes: 50µl, 100µl

Alternative Name: Phosphatidylethanolamine-binding protein 1; PEBP-1; HCNPpp; Neuropolypeptide h3; Prostatic-binding protein; Raf kinase inhibitor protein; RKIP; Hippocampal cholinergic neurostimulating peptide; HCNP; PBP; PEBP

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P30086

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: Raf kinase inhibitor protein (RKIP) is a cytosolic protein that was initially characterized as a phosphatidylethanolamine-binding protein (PBP) expressed in brain tissue and secreted from testis fluid. In addition, RKIP was identified by yeast two-hybrid screening of human T cell libraries directed at identifying proteins that associate with the BXB kinase domain of the serine/threonine kinase, Raf-1. Subsequent in vitro and in vivo studies indicate that RKIP binds to both the active and inactive forms of Raf-1 and thereby regulates the signaling cascade of the MAP kinase pathway. The specific association of RKIP with kinase-active Raf-1 competitively inhibits the binding and activation of the Raf-1 substrate MEK. RKIP, in turn, affects downstream MAP kinase signaling by decreasing the activation of MEK effector proteins, including ERK1 and ERK2, and the subsequent induction of AP-1 mediated transcription.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PEBP1 polyclonal antibody detects endogenous levels of PEBP1 protein.

Bioworld Molecular Weight: ~ 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of RKIP (L138) pAb at 1:500 dilution
Lane 1: 3T3-L1 whole cell lysate(40ug)
Lane 2: The Testis tissue lysate of Rat(40ug)
Lane 3: MCF-7 whole cell lysate(40ug)
Lane 4: L02 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 142-187 of Human PEBP1.

Conjugate: Unconjugated

Modification: Unmodified