Recombinant Human ERK3/MAPK12 Protein (His & GST Tag)

Size: 50μg

Catalogue Numbers: PKSH030318-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: ERK3; MAPK12

Target Synonym: ERK-6; ERK3; ERK6; MAPK12; P38GAMMA; PRKM12; SAPK-3; SAPK3; P38-gamma

UNIProt ID: P53778

Research Areas: Signal Transduction; Cell biology;

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Fusion Tag: N-His & GST

Accession: P53778

Background: ERK3; also known as MAPK12 and p38-gamma; belongs to theprotein kinase superfamily; CMGC Ser/Thr protein kinase family and MAP kinase subfamily. ERK3 is highly expressed in skeletal muscle and heart.ERK3 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly; p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK12 is required for the normal kinetochore localization of PLK1; prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.

Sequence: Met 1-Leu 367

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 69.8 kDa

Observed MW: 65 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only