Recombinant Human coronavirus (HCoV-HKU1) Nucleoprotein / NP Protein (His Tag)

Size: 100μg

Catalogue Number: PKSV030201-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: Nucleoprotein; NP

Target Symptom: coronavirus NP Protein; np Protein

Target Species: HCoV-HKU1

Expression Host: E.coli

Fusion Tag: N-His

Accession: YP_173242.1

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Sequence: Met1-Ala441

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM PB, 1M NaCl, pH 7.4, 22.5% Trehalose
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 43.21 kDa.

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only