ATPB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90110-50, BS90110-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06576(Human) P56480(Mouse) P10719(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebrafish

Applications: WB, IP, ICC/IF IHC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
IP:1:10-1:50

Background: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ATPB polyclonal antibody detects endogenous levels of ATPB protein.

Bioworld Molecular Weight: 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution.
Positive control: Line1: Hela Line2: HepG2 Line3: 293T, ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ATP5B, ATP 5B, ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide, ATP synthase subunit beta mitochondrial, ATP synthase subunit beta, mitochondrial, atp5b, ATPB, ATPB, ATPMB, ATPSB, Epididymis secretory protein Li 271, HEL-S-271, Mitochondrial ATP synthase beta subunit, Mitochondrial ATP Synthase Subunit Beta, Mitochondrial ATP synthetase beta subunit,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified