BNIP3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90143-50, BS90143-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q12983(Human) O55003(Mouse) EntrezGene:84480(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins. BNIP-3 preferentially binds to Bcl-xL and induces apoptosis by suppressing the anti-apoptosis activity of Bcl-xL.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: BNIP3 polyclonal antibody detects endogenous levels of BNIP3 protein.

Bioworld Molecular Weight: 21/30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of BNIP3 on different cells lysates using anti- BNIP3 antibody at 1/500 dilution.
Positive control:
Line 1: Human skeletal muscle
Line 2: Mouse kidney, ICC staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: BNIP3, BCL2 Adenovirus E1B 19kDa Interacting Protein 3, BCL2/adenovirus E1B 19 kDa protein interacting protein 3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP 3, BNIP3, BNIP3, NIP 3, NIP3,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified