MyoD1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90906-50, BS90906-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P15172(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IP, , FC

All Applications: WB:1:1,000-1:5,000
IP:1:10-1:50
FC:1:50-1:100

Background: Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either MyoD or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. MyoD transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. MyoD-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MyoD1 polyclonal antibody detects endogenous levels of MyoD1 protein.

Bioworld Molecular Weight: 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MyoD1 on Hela cells lysates using anti-MyoD1 antibody at 1/1,000 dilution., Flow cytometric analysis of Hela cells with MyoD1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Alternative Name: bHLHc1, Myf-3, MYOD1, bHLHc1, Class C basic helix-loop-helix protein 1, MYF 3, Myf-3, MYF3, Myoblast determination protein 1, Myod 1, MYOD, MYOD1, MYOD1, Myogenic differentiation 1, Myogenic factor 3, Myogenic factor MYF 3, Myogenin D1, PUM,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified