Recombinant Human IFN alpha2b

Size: 10ug

Catalogue Number: EPT205-10

Citations, Manuals and MSDS Available upon request.

Background: At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.

Alternate Names: Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A; LeIF A; IFNA2

Description: Recombinant Human Interferon Alpha-2b is produced by our E.coli expression system and the target gene encoding Cys24-Glu188 (Lys46Arg) is expressed.

Accession: P01563

Molecular Weight: 19.4 KDa

Apparent Molecular Weight: 17 KDa, reducing conditions

Storage Condition: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Biological Activity: Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED50 for this effect is 5 ng/mL.

Redissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Research Use Only