COASY Polyclonal Antibody

Catalogue Numbers: BS60053-50, BS60053-100

Sizes: 50µl, 100µl

Alternative Name: Bifunctional coenzyme A synthase; CoA synthase; NBP; POV-2; Phosphopantetheine adenylyltransferase; Dephospho-CoA pyrophosphorylase; Pantetheine-phosphate adenylyltransferase; PPAT; Dephospho-CoA kinase; DPCK; Dephosphocoenzyme A kinase; DPCOAK; COASY; PSEC0106

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13057

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: COASY (coenzyme A synthase), also known as NBP (nucleotide binding protein), DPCK (dephospho-coenzyme A kinase), PPAT (pantetheine-phosphate adenylyltransferase), UKR1 or pOV-2, is a bifunctional enzyme involved in the biosynthesis of coenzyme A (CoA). COASY exhibits both Ppat activity and DPCK activity, catalyzing steps four and five, respectively, of the CoA biosynthetic pathway. Functioning as a widely expressed monomer and induced by phospholipids, COASY localizes to the outer mitochondrial membrane and facilitates the conversion of 4'-phosphopantetheine to dephospho-CoA and the subsequent generation of CoA. CoA is an important molecule in the cell, participating in carbohydrate, amino acid and fatty acid metabolism. It is the predominant acetyl and acyl group carrier and is used as a substrate by approximately 4% of all cellular enzymes. Due to alternative splicing events, an additional isoform of COASY, namely COASY b, is expressed in brain and contains an extra 29 amino acids at the N-terminus.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: COASY polyclonal antibody detects endogenous levels of COASY protein.

Bioworld Molecular Weight: ~ 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of COASY polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human COASY.

Conjugate: Unconjugated

Modification: Unmodified