CD20 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9888M-50, BS9888M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11836

Host: Rabbit

Reactivity: Human, Monkey

Applications: WB, IHC

All Applications: WB: 1:1000-1:5000
IHC: 1:50-1:200

Background: CD20 is a leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. The cytoplasmic domain of CD20 contains multiple phosphorylation sites, leading to additional isoforms. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. CD20 functions as a calcium-permeable cation channel, and it is known to accelerate the G0 to G1 progression induced by IGF-1. CD20 is activated by the IGF-1 receptor via the alpha subunits of the heterotrimeric G proteins. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of CD20 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD20 Rabbit mAb at 1:1000 dilution
Lane 1: Raji whole cell lysate, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD20 antibody. Counter stained with hematoxylin.

Alternative Name: B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD20; MS4A1; CD20

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified