PODN Polyclonal Antibody

Catalogue Numbers: BS60696-50, BS60696-100

Sizes: 50µl, 100µl

Alternative Name: Podocan; PODN; SLRR5A; UNQ293; PRO332

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7Z5L7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Non-collagenous proteins of the small leucine-rich repeat (SLR) protein family are considered important components of the extracellular matrix. The extracellular matrix plays an integral role in the pivotal processes of development, tissue repair and metastasis by regulating cell proliferation, differentiation, adhesion and migration. Podocan, also known as PCAN, SLRR5A or PODN, is a 613 amino acid secreted protein belonging to the small leucine-rich proteoglycan (SLRP) family and the class V subfamily. Expressed in kidney, heart, liver, pancreas and vascular smooth muscle cells, Podocan exists as three alternatively spliced isoforms containing twenty LRR (leucine-rich repeats), a unique N-terminal cysteine-rich cluster pattern and a highly acidic C-terminal domain. Podocan is considered a glycoprotein containing N-linked oligosaccharides that may be involved in growth regulation in cardiovascular tissues. Encoded by a gene located on human chromosome 1, Podocan interacts type I collagen (COL1).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PODN polyclonal antibody detects endogenous levels of PODN protein.

Bioworld Molecular Weight: ~ 69 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PODN polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human PODN.

Conjugate: Unconjugated

Modification: Unmodified