TNFAIP3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91360-50, BS91360-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21580(Human) Q60769(Mouse) M0R7V5(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: A20 is a Cys2/Cys2 zinc finger protein that is induced by a variety of inflammatory stimuli and regulates gene expression. Specifically, A20 is induced by tumor necrosis factor (TNF) and interleukin 1 (IL-1), and acts as a negative regulator of nuclear factor κ B (NFκB) gene expression. By inhibiting NFκB activation, A20 plays a critical role in terminating NFκB responses to various stimuli. Although the C-terminal region of A20 contains seven zinc finger domains, only four of these domains are required for in vitro inhibition of TNF-induced NFκB activation. A20 also interacts with several other proteins, such as TRAF2, TRAF6 and IkB kinase (IKK) γ protein, and can thereby inhibit cell death. TXBP151, a novel A20-binding protein, may mediate the anti-apoptotic activity of A20. Involved in the negative feedback regulation of signal transduction, A20 and A20-binding proteins may be useful as novel therapeutic tools in the treatment of a variety of diseases.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TNFAIP3 polyclonal antibody detects endogenous levels of TNFAIP3 protein.

Bioworld Molecular Weight: 90 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TNFAIP3 on Jurkat (1) and Daudi (2) cell lysate using anti-TNFAIP3 antibody at 1/100 dilution., ICC staining TNFAIP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TNF alpha-induced protein 3, TNFAIP3, OTUD7C, A20, AISBL, MGC104522, MGC138687, MGC138688, OTU domain containing protein 7C, OTU domain-containing protein 7C, OTUD7C, Putative DNA binding protein A20, Putative DNA-binding protein A20, TNAP3, TNF alpha-induced protein 3, TNFA1P2, TNFAIP 3, TNFAIP3 (A20), TNFAIP3, Tumor necrosis factor alpha induced protein 3, Tumor necrosis factor alpha-induced protein 3, Tumor necrosis factor induced protein 3, Tumor necrosis factor inducible protein A20, tumor necrosis factor, alpha-induced protein 3, Zinc finger protein A20,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified