Kininogen 1 (KNG1) Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6646-50, BS6646-100

Swiss-Prot: P01042

Host: Rabbit

Kininogen 1 (KNG1) Polyclonal Antibody-BS6646

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 72KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Rat lung, using Kininogen1 antibody at 1:490 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of HepG2 cells using Kininogen 1 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: KNG1; BDK; BK; KNG

Immunogen: Recombinant fusion protein of human Kininogen 1 (KNG1)(NP_001095886.1).

Modification: Unmodified

Conjugate: Unconjugated