CX3CR1 Polyclonal Antibody

Catalogue Numbers: BS60627-50, BS60627-100

Sizes: 50µl, 100µl

Alternative Name: CX3C chemokine receptor 1; C-X3-C CKR-1; CX3CR1; Beta chemokine receptor-like 1; CMK-BRL-1; CMK-BRL1; Fractalkine receptor; G-protein coupled receptor 13; V28; CX3CR1; CMKBRL1; GPR13

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49238

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Chemokines are chemoattractant proteins that are divided into subfamilies based upon cysteine signature motifs termed C, CC, CXC and CX3C. Fractalkine, also designated CX3CL1, contains the CX3C motif and is widely ex-pressed in brain and upregulated in endothelial cells in response to inflammatory signals, such as LPS, IL-1, TNF and CD40L. Fractalkine exists both as a membrane-bound form and as a chemotactic soluble form, and binds its cognate receptor, CX3CR1, with high affinity, to induce leukocyte adhesion and migration or chemotactic functions. CX3CR1, previously designated V28 and chemokine β receptor-like 1 (CMKBRL1), is expressed in neutrophils, monocytes, T lymphocytes and several organs including brain. CX3CR1 also functions with CD4 as a co-receptor for the HIV-1 virus envelope protein, and patients homozygous for a variant haplotype of CX3CR1 progress to AIDS more rapidly than those with other haplotypes. CX3CR1 may also be involved in the pathogenesis of atherosclerotic coronary artery disease (CAD) and is considered a potential drug target for therapeutic intervention of endothelium-related inflammatory diseases.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CX3CR1 polyclonal antibody detects endogenous levels of CX3CR1 protein.

Bioworld Molecular Weight: ~ 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CX3CR1 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human CX3CR1

Conjugate: Unconjugated

Modification: Unmodified