hnRNP K (Y280) Polyclonal Antibody

Catalogue Numbers: BS2997-50, BS2997-100

Sizes: 50µl, 100µl

Alternative Name: Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP; HNRNPK; HNRPK

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P61978

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: hnRNP K (Y280) polyclonal antibody detects endogenous levels of hnRNP K protein.

Bioworld Molecular Weight: ~ 58-62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of hnRNP K (Y280) pAb at 1:1000 dilution
Lane 1: HCC827 whole cell lysate(40ug)
Lane 2: HEK293T whole cell lysate(20ug)
Lane 3: A549 whole cell lysate(40ug)
Lane 4: C6 whole cell lysate(40ug)
Lane 5: CT26 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human hnRNP K.

Conjugate: Unconjugated

Modification: Unmodified