Amphomycin is a natural antibacterial lipopeptide. Cyclic lipopeptides are a promising class of natural products with antibiotic properties. Cyclic lipopeptides are amphiphilic molecules, composed of a fatty acid tail linked to a short oligopeptide which form a macrocylic ring structure. In vitro: In previous study, Calf brain endoplasmic reticulum membranes were incubated with varying concentrations of GDP-mannose in the presence and absence of amphomycin, results showed no significant difference in apparent Km for GDP-mannose. However, the Vmax was reduced in the presence of amphomycin as compared with in its absence. Moreover, when mannosylphosphoryldolichol synthase activity was measured in the presence of amphomycin, the shape of the substrate velocity curve changed from a rectangular hyperbola to a sigmoid . In vivo: The PK of lipopeptides, the semi-synthetic amphomycin analogues, were evaluated in mice and rats following single i.v. and oral administration. Following oral administration at 50 mg/kg, plasma concentrations of amphomycin analogues were <0.3-0.9 μg/mL, indicating that oral availability was low. Following i.v. administration (5-10 mg/kg), the majority of lipopeptides demonstrated a long half-life, low clearance and a volume of distribution indicative of extracellular penetration. The long half-life and low clearance indicated that drug serum concentrations remained above the target minimal inhibitory concentration levels for significant periods of time. When combined with the potent efficacy against Gram-positive organisms, the results supported further development of these lipopeptide analogues towards clinical evaluation . Clinical trial: Up to now, amphomycin is still in the preclinical development stage.

Product information

CAS Number: 1402-82-0

Molecular Weight: 1290.42

Formula: C58H91N13O20

Chemical Name: (3S)-3-{[(3S,4R,7S,13S,16R,22S,28S,31S,34R)-16-[(1R)-1-aminoethyl]-31-[(1S)-1-carboxyethyl]-22,28-bis(carboxymethyl)-4-methyl-2,6,12,15,18,21,24,27,30,33-decaoxo-13-(propan-2-yl)-1,5,11,14,17,20,23,26,29,32-decaazatricyclo[32.4.0.0⁷,¹¹]octatriacontan-3-yl]carbamoyl}-3-[(3Z)-10-methyldodec-3-enamido]propanoic acid

Smiles: CC(C)[C@@H]1NC(=O)[C@H](NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H]2CCCCN2C(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)C/C=C\CCCCCC(C)CC)[C@@H](C)NC(=O)[C@@H]2CCCN2C1=O)[C@H](C)C(O)=O)[C@@H](C)N

InChiKey: WAFOSUDOWLQGBG-NUZIGYSUSA-N

InChi: InChI=1S/C58H91N13O20/c1-8-30(4)18-13-11-9-10-12-14-21-39(72)63-36(26-44(79)80)51(83)69-48-33(7)62-52(84)38-20-17-23-71(38)56(88)45(29(2)3)67-55(87)47(32(6)59)66-41(74)28-61-49(81)34(24-42(75)76)64-40(73)27-60-50(82)35(25-43(77)78)65-54(86)46(31(5)58(90)91)68-53(85)37-19-15-16-22-70(37)57(48)89/h12,14,29-38,45-48H,8-11,13,15-28,59H2,1-7H3,(H,60,82)(H,61,81)(H,62,84)(H,63,72)(H,64,73)(H,65,86)(H,66,74)(H,67,87)(H,68,85)(H,69,83)(H,75,76)(H,77,78)(H,79,80)(H,90,91)/b14-12-/t30?,31-,32+,33+,34-,35-,36-,37+,38-,45-,46-,47+,48-/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

Products are for research use only. Not for human use.