CAPNS2 Polyclonal Antibody

Catalogue Numbers: BS60567-50, BS60567-100

Sizes: 50µl, 100µl

Alternative Name: Calpain small subunit 2; CSS2; Calcium-dependent protease small subunit 2; CAPNS2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96L46

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Calpain family of proteins are calcium-regulated thiol proteases which have broad endopeptidase activity throughout the body. Calpain small subunit 2, also known as CSS2 or CAPNS2, is a calcium-dependent protease that is expressed ubiquitously in the cytoplasm. Part of a heterodimer composed of a small subunit and a large subunit, CSS2 catalyzes proteolysis of various proteins involved in cytoskeletal remodeling and signal transduction. CSS2 also acts as a chaperone to the larger subunit, mediating its correct folding and conformation. When bound as a heterodimer, CSS2 is thought to keep the catalytic activity of the large subunit dormant. After binding calcium, CSS2 is released from the complex, thereby activating the large subunit and allowing CSS2 to translocate from the cytoplasm to the cell membrane. Defects in the gene encoding CSS2 result in incorrect calpain activity and retarded fetal development, suggesting that CSS2 expression is essential for proper growth.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CAPNS2 polyclonal antibody detects endogenous levels of CAPNS2 protein.

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CAPNS2 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human CAPNS2

Conjugate: Unconjugated

Modification: Unmodified